KMID : 0880220120500010155
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Journal of Microbiology 2012 Volume.50 No. 1 p.155 ~ p.160
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Acinetobacter baumannii outer membrane protein a modulates the biogenesis of outer membrane vesicles
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Moon Dong-Chan
Choi Chul-Hee Lee Jung-Hwa Lee Je-Chul Choi Chi-Won Kim Seung-Il Kim Hye-Yeon Park Jeong-Soon
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Abstract
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Acinetobacter baumannii secretes outer membrane vesicles (OMVs) during both in vitro and in vivo growth, but the biogenesis mechanism by which A. baumannii produces OMVs remains undefined. Outer membrane protein A of A. baumannii (AbOmpA) is a major protein in the outer membrane and the C-terminus of AbOmpA interacts with diaminopimelate of peptidoglycan. This study investigated the role of AbOmpA in the biogenesis of A. baumannii OMVs. Quantitative and qualitative approaches were used to analyze OMV biogenesis in A. baumannii ATCC 19606T and an isogenic ¥ÄAbOmpA mutant. OMV production was significantly increased in the ¥ÄAbOmpA mutant compared to wild-type bacteria as demonstrated by quantitation of proteins and lipopolysaccharides (LPS) packaged in OMVs. LPS profiles prepared from OMVs from wild-type bacteria and the ¥ÄAbOmpA mutant had identical patterns, but proteomic analysis showed different protein constituents in OMVs from wild-type bacteria compared to the ¥ÄAbOmpA mutant. In conclusion, AbOmpA influences OMV biogenesis by controlling OMV production and protein composition.
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KEYWORD
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outer membrane protein A, lipopolysaccharides, peptidoglycan, protein sorting
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